Characteristics and introduction of 1--silicone oil medium objective lens of Olympus high-end objective lens series

Olympus high-end objective lens series - silicone oil medium objective lens

With the continuous development of microscopic technology, users have higher and higher requirements for image quality and detail. The importance of objective lens as the core of microscopic optical system is unquestionable. In recent years, Olympus has developed several special objective lenses to meet the special needs of users for different applications to meet the experimental requirements of researchers.

Olympus launched the silicone oil objective lens UPLSAPO30XS and UPLSAPO60XS in the second half of 2010. This is the first silicone oil medium objective lens for live cell imaging, which significantly improves live cell confocal, wide-field fluorescence. , multiphoton, DIC and other live cell experiments.

The new silicone oil objective has significant advantages over traditional oil and water mirrors in deep and long-term imaging. Compared with oil mirrors, because the refractive index of silicone oil is very close to that of living cells/tissue (about 1.36- 1.38), it can minimize the negative effects of refractive index mismatch and spherical aberration, so it can help users to capture Liver specimens are brighter, higher resolution images and can even penetrate deep into cells and tissues.

Currently, in long-term time series experiments, glycerol-based oil mirrors are often used for imaging. But glycerin absorbs moisture from the air and changes its refractive index over time. In contrast, the new silicone oil objective is better in long-term imaging trials. Because the silicone oil medium is very stable at 37 ° C, and it does not cause drying and hardening due to evaporation. Therefore, it is not necessary to add mirror oil during the long-time delay observation process, and the stability of long-term observation can be ensured.