DON primarily its toxigenic Fusarium (of Fusarium) Jurassic bacteria, mainly contaminated corn, wheat, rice, barley, millet and oat grains. DON has estrogenic effects, a major role in the reproductive system, will enable livestock, poultry and other produce estrogen hyperthyroidism. Various pastas made from vomiting toxin- containing flour can also cause symptoms of central nervous system poisoning, such as nausea, chills, headache, mental depression and ataxia. Pregnancy animals ( including people ) eating foods containing vomiting toxin can cause miscarriage, stillbirth and teratogenesis , so establishing a rapid, sensitive and accurate vomiting toxin detection technology is of great significance to human health.
At present, the detection methods of vomiting toxin include liquid chromatography, enzyme-linked immunoassay, and immunocolloidal gold.
(1) hapten deoxynivalenol (Synthesis Route technology see below)
DON 20-100mg, in 2-5m l of toluene, and the reaction was stirred at 80 deg.] C maleic anhydride 1-3mol After 8-20h, the solvent was evaporated, acidified, extracted, and recrystallized to give DON hapten.
( 2 ) vomiting toxin full antigen synthesis
The vomiting toxin hapten is conjugated to bovine serum albumin to obtain an immunogen.
Take 60mg bovine serum albumin were dissolved in 9.5ml PBS (0.01mol / L, pH5.0 ) was added 12.5mgEDC, 1mlDMF added 10mg dissolved DON hapten, IH stirred at room temperature, plus 6mg EDC, was stirred in the dark The reaction was carried out overnight, dialyzed against 0.01 mol/L PBS for 3 days, and dialyzed three times a day to obtain an immune antigen of vomiting toxin and bovine serum albumin.
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