Many pathogens do not show obvious symptoms to fish, so it is obviously not sufficient to rely solely on visual inspection. It is also necessary to combine microscopic examination to determine the pathogen. There are two ways to check.
Slide compression method: Using two glass slides with a thickness of 3-4 mm and a size of about 6 cm and 12 cm, first remove the scraped surface mucus, intestinal contents, and internal organs, and place them on a slide. Add an appropriate amount of fresh water or saline (in vitro organ or mucus with water; intracorporeal organs, tissues or contents with 0.65% saline solution), press it into a transparent thin layer with another glass slide and place it on the dissection microscope Or under a low magnification microscope, if pathogens or certain suspicious conditions are found, carefully remove them from the thin layer with tweezers or dissection needles, micropipettes, and place them in petri dishes containing fresh water or salt water. Observe and deal with it.
Slide compression method: Use small scissors or tweezers to remove a small drop of material or a small piece of tissue on a glass slide, add a small drop of water or saline, cover the coverslip, gently flattened First, under a low magnification microscope, when parasites or suspicious phenomena are detected, they are carefully examined with a high magnification microscope.
At the time of microscopic examination, viral pathogens can be observed only by electron microscopy. Common bacterial pathogens can be detected under light microscopy. However, depending on the observation form, it is not yet possible to determine their types. If necessary, isolates, pure cultures, and infection experiments are needed to determine the pathogen.
Meltblown Cloth KN95 Face Mask
Kn95 Protective 3D Mask,Kn95 5 Layers Respirator,Meltblown Cloth Kn95 Face Mask,Kn95 5 Layers Fold Flat Face Mask
Jiangmen anjian biotechnology co. LTD , https://www.anjianmask.com