Canadian invention of rapid detection of bacterial drug resistance microdevices

Release date: 2016-11-24

Recently, the University of Alberta in Canada reported that the university's engineering and pharmaceutical researchers have invented a device that can quickly identify drug-resistant bacteria, which can be used to find specific antibiotics that are most effective against restraint bacteria.

This project invention differs from the often time-consuming method of detecting bacterial cultures, which is a nanotechnology-based microdevice that can be used to quickly obtain results.

A prominent feature of this device is that it resembles the cantilever of a diving board, the surface of which etches a microfluidic channel that is one-fifth the width of the hair strand. The channel is coated with an antibody-like biomaterial, and harmful bacteria such as E. coli or Listeria in the fluid sample can be attached to the surface of the channel.

The cantilever on the device absorbs bacteria into the channel and causes changes in the cantilever resonance frequency (mass) and cantilever deflection (adsorption pressure). The bacteria are irradiated with infrared light, and the degree of cantilever bending is proportional to the case where the bacteria absorb light, thereby providing a nano-infrared spectrum for selective recognition. By observing the change in the intensity of the cantilever micro-vibration, you can discover the life and death of the bacteria, and then know which antibiotic is most effective against bacteria.

Researchers hope to find ways to combat bacterial resistance, preventing or at least reducing the spread of resistant strains. The device can perform multiple tests in a short period of time, thus quickly identifying bacteria that are resistant to antibiotics.

The device can be used to detect particularly small fluid samples, which can be a millionth of a raindrop. The size of the device is an advantage when people only need a very small sample, such as in a neonatal intensive care unit or when only very small samples are available.

The team has applied for a patent for the technology. This approach will provide a platform for developing portable, high-throughput devices that detect bacteria's response to antibiotics in real time.

The research results of the project invention were published in the October 2016 issue of Nature Communications.

Source: Ministry of Science and Technology website

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