With the rapid development of proteomics, the use of recombinant proteins has increased dramatically in recent years. Recombinant hybrids containing an affinity tag such as GST, Myc, His, etc., can be used to aid in the purification of the target protein, which has been widely used. The use of fusion proteins to facilitate the purification and detection of recombinant proteins is somewhat recognized. The Myc tag is equivalent to the human c-Myc 410-419 peptide (sequence: EQKLISEEDL). The Myc tag can be placed at the C-terminus or the N-terminus, but the low pH elution conditions of the Myc recombinant protein tend to reduce protein viability, so the Myc tag system is widely used for detection but is rarely used for purification. The Myc tag has been successfully used in WB hybridization, immunoprecipitation, and flow cytometry. It can therefore be used to detect the expression of recombinant proteins in bacteria, yeast, insect cells and mammalian cells.
How to choose the right Myc tag antibody?
When choosing a label antibody, we mainly rely on the needs of our own experimental application. Factors to be considered here include the type of detection of the Myc antibody, the cloning of the Myc antibody, and the source of preparation. For example, can the Myc antibody be used for the detection of immunofluorescence IF? Can it be used in Western Bloting and immunoprecipitation IP experiments? Do you need a monoclonal Myc tag antibody with better specificity?
In general, the more types of applications, the greater the choice in use, and the monoclonal antibodies from mouse sources generally have better specificity, stability and potency than polyclonal antibodies, while polyclonal antibodies It has the advantage of higher affinity. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl of Myc antibody (if the dilution ratio of WB is 1:5,000, the final solution is equivalent to 500 ml), 1 ml of Myc antibody with lower potency (if the dilution ratio of WB test is 1:200) The final use solution is equivalent to 200ml). Therefore, when selecting antibodies, not only the amount of antibody needs to be considered, but also the titer of the antibody, that is, the recommended dilution ratio for different applications.
Abbkine's Myc tag antibody, I used to do immunoprecipitation experiments, the effect is not bad, compared with the previous SSS (another brand, the name is hidden), the titer is much higher, and the stability is also very Not bad. Because once, after half a year later, the effect is still very good, because the price is also very cheap (we were looking for Abbkine custom 1ml large package), and the laboratory has been using it. ——Huazhong Agricultural College Students
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| Product name and description | Brand | Item number | product manual |
| Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | Abbkine | A02060 | Details |
| HRP Conjugated Anti-Myc Tag Monoclonal Antibody (2D5) | Abbkine | A02065 | Details |
| Anti-Myc Tag Rabbit Polyclonal Antibody | Abbkine | A02061 | Details |
Why choose Abbkine's Myc tag antibody?
The Myc antibodies currently available on the market are domestically packaged or manufactured; they are also imported, such as Abcam, CST, Sigma, Novus, Millipore, etc. The domestically packaged Myc antibody is insufficient in quality stability, and its valence is also lost after being packaged. However, some of the Myc antibodies originating in domestic laboratories have better detection activity, but there are gaps in the application type, and it is difficult to control the stability between batches, and only a small amount of domestically produced antibodies are also available in China. Polyclonal antibody. In the imported brands, we have selected the following products for comparison of price and application type:
| Brand | Item number | 2013 offer | specification | App types |
 | Ab6046 | ¥4418 | 100μl | ICC/IF, IHC, WB |
 | 2272S | ¥2997 | 100μl | WB, IF, FC |
 | 05-419 | ¥3732 | 200μg | WB, IF, IC |
 | A02060 | ¥1800 | 200μl | WB, IF, IP |
Therefore, we recommend to you Abbkine's Myc tag antibody Anti-Myc Tag Monoclonal Antibody (2D5) (Cat. No. A02060). The Myc antibody is a monoclonal antibody and the clone number is 2D5. The antigen is a KLP-coupled c-Myc 410-419 peptide (sequence: EQKLISEEDL), ie Myc tag protein as an immunogen. The Myc monoclonal antibody has a very high titer, and the WB experiment recommends a dilution ratio of 1:1000-1:10000, and even customers report higher dilution ratios. It also performed well in immunofluorescence IF and immunoprecipitation IP experiments. In addition, Abbkine also provides HRP-conjugated Myc-tagged antibodies, especially for IP-WB verification to remove secondary antibody interference; rabbit-derived polyclonal Myc antibody also provides customers with another Myc-label detection protocol.
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| Product name and description | Brand | Item number | product manual |
| Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | Abbkine | A02060 | Details |
| HRP Conjugated Anti-Myc Tag Monoclonal Antibody (2D5) | Abbkine | A02065 | Details |
| Anti-Myc Tag Rabbit Polyclonal Antibody | Abbkine | A02061 | Details |
Abbkine also offers large packaging of 1ml and larger Myc-labeled antibodies, making it ideal for routine testing of the immunological detection of the Myc tag system. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.
The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its China. The regional agent will provide customers with the most cost-effective routine immunological reagents, such as various tag antibodies, internal reference antibodies, conventional primary antibodies and a full range of secondary antibodies, antibody detection substrates and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
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