Millipore Kit recommends using the Q800R3 device to cut chromatin

Chromatin is generally considered to be a complex composed of DNA, histones, and non-histones. The components of the RNA chromatin complex are thought to consist of mRNA or traditional snRNA, which is transiently associated with chromatin during transcription. However, there is increasing evidence that various types of non-coding RNAs (eg, long-chain non-coding RNAs, enhancer RNAs, and even miRNAs) bind to chromatin and may hybridize by sequence-specific and/or through structure and space. Mechanism to achieve the adjustment function. To better describe these regulatory mechanisms, a method is needed to identify and describe the interaction between RNA molecules (both coding and non-coding), proteins, and DNA.

Magna ChIRP ^TM (Download Instructions) by Millipore (Merck - Millipore) introduced for research and lncRNA (long chain non-coding RNA) commercial kits chromatin interaction DNA sequence and protein. This kit uses a unique method to find binding sites for chromatin-associated RNA (such as lncRNA) to genomic DNA sequences, as well as proteomic analysis of protein components of lncRNA regulatory complexes.

The study of chromatin or chromatin RNA complexes is inseparable from ultrasonic shears. Millipore (Merck - Millipore) recommended Qsonica ChIRP ^TM use in manual Q800R3 chromatin / DNA to complete shear sonicator chromatin trim.

Screenshot of the inside of the Kit manual:


The basic principle of Magna ChIRP ^{TM is} as follows: