Bottled fermented material is one centimeter tall
About 4 cm from the bottle mouth above the medium, a 1-2 cm fermentation material was loaded and sterilized. Because the fermented material itself is sterile, the mycelium quickly fills the entire top of the bottle after the bacteria is introduced, and the mycelium eats at the same time. The advantage of this method is that the bacteria are not easy to infect the culture medium, and the strains have the same age. The pollution rate can be reduced to less than 3%.
Wheat germination
Allow the wheat germ to germinate before bottling. The storage material of wheat grain mainly includes starch, fat and protein. When the seed is swelled and the physiological activity of cells is reactivated, the activities of various enzymes are enhanced. These substances are decomposed into simple structures such as maltose, sucrose, amino acids and the like at the time of germination. It is easy to be used by mycelium and the mycelium grows fast. The specific approach is: one-time use of bacteria to germinate seed germination, bud length of 1-2 times the length of wheat, fully dried after storage, wait until the day before doing bacteria, soak the wheat 12h, directly bottled Sterilization. The advantage of this method is that it saves the process of cooking wheat grains, saves manpower and financial resources, and has fast germs and less bacteria. The pollution rate is only 2%-3%.
Use liquid bacteria
Liquid strains overcome the disadvantages of solid strains and liquid strains can be cultivated directly as cultivars. The use of liquid strains not only has a low probability of infestation by the bacteria, but the pollution rate can be reduced to about 2%. Moreover, from the long-term perspective, because it does not use plastic bags, it does not pollute the environment and reduces the production cost of strains. The bottom of the original kind of bottle
Start inoculation from the bottom of the original bottle
We know that the species of bacteria in the lower part of the original species has a short bacteria age, strong vitality, and less germs, and the hyphae are quick to eat after inoculation. The pollution rate is low, and the pollution rate can be reduced to about 3%. In order to further reduce the contamination rate of cultivars, it is recommended that one-fifth of the original species in the upper part of the bottle be discarded to prevent contamination of cultivars by bacteria that are invisible to the naked eye.
The time of inoculation of bacteria in the hot season is advanced or delayed
In the period of July-August, the temperature is low, such as morning or evening. Of course, if the bacteria in the room with empty leaves is better, the pollution rate can drop below 5%.
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