High-speed refrigerated centrifuge main use and experimental reference speed

The speed of the high-speed refrigerating centrifuge can reach 10,000 rpm to 30,000 rpm. With the performance and structure of the refrigerated centrifuge, the angle turret used in the high-speed refrigerating centrifuge is mostly made of titanium alloy or aluminum alloy. The centrifuge tube is a polyethylene hard plastic product with a cover. Such centrifuges are often used to collect microorganisms, cell debris, cells, large organelles, sulfate precipitates, and immunoprecipitates.

Desktop high speed refrigerated centrifuge

Desktop intelligent high-speed refrigerated centrifuge 3H24RI, maximum speed 24000rpm

High-speed refrigerated centrifuge construction <br> The centrifuge consists of a housing, a centrifugal chamber, a rotor, a freezer and a control circuit.

<br> instrument classification can be divided into desktop high-speed refrigerated centrifuge and vertical high-speed refrigerated centrifuge (also called floor-speed refrigerated centrifuge).

The high-speed refrigerated centrifuge and its ancillary equipment (rotary heads and others) are mainly used for the following experiments from the use, with reference to the speed:

1. Cell precipitation, cell density gradient centrifugation, cell flotation purification; (3,000 ~ 12,000 rpm)


2. Subcellular structures (nuclear, plasma membrane, mitochondria, endoplasmic reticulum, lysosomes, Golgi, microsomes, microtubules, ribosomes, cell bodies, microperoxidases, centrosomes, microfilaments, etc. Precipitation and density gradient centrifugation, differential purification, etc.; (10,000 rpm ~ 21,000 rpm)


3. Alcohol precipitation of biomacromolecules (proteins, nucleic acids, ribosomes, polysomes, chromosomes, polysaccharides and peptides), ammonium sulfate precipitation, phenol precipitation; sucrose or glycerol gradient centrifugation; (15,000~22,000 rpm)


4. Pretreatment of biomacromolecules for ultra-fast purification; (15,000 rpm to 20,000 rpm)


5. Separate and purify the protein, DNA, RNA, plasmid and small-capacity extraction of the above components using the kit. (8,000 rpm~15,000 rpm)


6. Precipitation of the virus, PEG precipitation of the virus, density gradient centrifugation of the virus; (15,000 rpm to 20,000 rpm)


7. Small-capacity pilot and large-capacity fermentation products, cell culture, collection and concentration of bacteria and yeast, collection and purification of cracked products. (4,000rpm~18,000rpm)


8. Collection and concentration of whole virus, lytic virus protein, expression cells and cells during vaccine production. (Use fixed angle rotor or high speed continuous flow head); (10,000 rpm ~ 18,000 rpm)


9. Biological products, chemical polymer products, nano materials, pretreatment of drugs. (5,000rpm~18,000rpm)

Floor-standing high-speed refrigerated centrifuge

HR/T20MM vertical high speed refrigerated centrifuge maximum speed (r/min): 20600

10. PCR matching and fragmentation of biomacromolecules. (8,000~12,000rpm)


11. Centrifugal separation of tissue culture fluid (cone bottom or round bottom tube); (3,000~15,000 rpm)


12. Concentration of animal and plant homogenate and medium purity separation and purification, differential centrifugation of the precipitate, concentration and density gradient centrifugation of the supernatant; (5,000 rpm to 20,000 rpm)


13. Separation of blood products: blood cell sedimentation, plasma protein precipitation, blood component separation, blood cell separation, and flotation purification of blood cells; (3,000 to 20,000 rpm)


14. Clinical diagnosis: centrifugation of body fluids, excretions, tissues, and centrifugation of samples required for clinical diagnosis. (3,000~15,000rpm)


15. Immunological research: Centrifugal separation of multi-tube samples in standard microplates and deep well plates in radioimmunoassay and enzyme-linked immunosorbent assays. (3,000~5,000rpm)


16. Biomacromolecules, nucleic acid samples, microplates, deep well plate sediment collection. (3,000~10,000rpm)


17. Solid-liquid separation of soil and minerals; (11,000 rpm)


18. Oil and water separation of crude oil, separation of oil and solid particles and other studies; (3,000~10,000 rpm)


Conclusion: From the point of view of application, the highest design speed of high-speed refrigerated centrifuge is below 30,000 rpm, which can meet the comprehensive needs of various users.

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