Carbon dioxide incubator is an advanced instrument for cell, tissue and bacterial culture. It is a key equipment for immunology, oncology, genetics and bioengineering. It is widely used in microbiology, medicine, pharmaceutical, environmental protection, food, animal husbandry, etc. Research and production in the field of science. The principle is to artificially cause the artificial environment of growth and reproduction of microorganisms and cells, bacteria and viruses in the incubator, such as controlling certain temperature, humidity, gas, etc., mainly for tissue culture and cultivation of some special microorganisms. Carbon dioxide incubator is one of the commonly used equipment for cell culture. It is mainly used for animal cell culture. It can maintain a certain concentration of carbon dioxide to simulate the growth environment of living organisms. It is used to cultivate some primary cells and passages that are harsh on environmental conditions. Cells." However, the culture of animal cells is a sputum that requires oxygen. The "carbon dioxide incubator" mentioned in "conditions are demanding" is insulated by water jacket and air jacket. The air jacket type uses an insulation system and a surface heat dissipating component to equalize the temperature inside the incubator. However, because the external electric heating system is unevenly distributed, the temperature in the incubator may not be uniform, and the temperature difference is relatively large. The water jacket type requires the user to inject a large amount of distilled water into the space of the tank wall when the incubator is installed. Since the specific heat capacity of water is very high, the advantages of the water jacket type are to help uniform heat dissipation and avoid formation of a cold zone, and to better reduce the temperature drop in the incubator after power failure. The water jacket type has a good temperature uniformity due to the water circulation for 4 weeks. Most of the new CO2 incubators are water jacketed.
The working principle of carbon dioxide incubator:
First, the temperature control:
1. The temperature controller consists of Pt100 platinum resistance as the sensor and digital control circuit, LED digital display circuit, etc. When the sensor output is proportional to the temperature, the resistance signal is converted into a voltage signal and amplified by the circuit, and sent to the display circuit for display. temperature.
2. The other way is sent to the comparator and compared with the set value. When the two are biased, the thyristor power tube output power is triggered to generate heat in the heating tube. When the deviation is reduced to zero, the heat generated by the heating tube is also reduced until the heating is stopped.
3. There is over temperature and water level alarm function in the control line. When the display temperature exceeds the set value of 2.0 °C, the temperature controller emits an audible and visual alarm signal, and at the same time cuts off the output, stops heating, prevents the temperature from rising continuously; when the water level in the water tank is too high or too low, the water level controller emits an audible and visual alarm. Signal while cutting off the heating output. Only the water jacketed carbon dioxide incubator has a water level alarm function!
Second, the control of the gas path:
1, gas routing high concentration of carbon dioxide cylinders, air pumps, pressure regulators, needle valves, solenoid valves, flow meters and gas cylinders. (High-concentration carbon dioxide cylinders are provided by the user).
2. When a certain concentration of high-concentration carbon dioxide gas is input, the flow rate and time control of the regulating valve, the needle valve and the solenoid valve are controlled to ensure that a certain amount of carbon dioxide gas enters the working chamber, and the carbon dioxide concentration value in the working chamber is automatically controlled. This value is simultaneously displayed on the display circuit for easy observation. In order to facilitate the user to monitor and sample the concentration during use, a sampling and monitoring port is installed on the back side of the carbon dioxide incubator. Due to the adoption of the fast and slow double inflation method, after the carbon dioxide incubator is opened and closed, the concentration of carbon dioxide in the working chamber can be quickly recovered without overshoot.
3. During the working process of the carbon dioxide incubator, the working chamber is ventilated by the gas storage bottle to maintain a stable carbon dioxide concentration value. Third, the humidity control:
This machine is equipped with a water tray when the culture needs to maintain a certain humidity in the working chamber. The user can put the water tray into the working chamber during the cultivation, and the water tray is added with the appropriate amount of distilled water to naturally evaporate in the working chamber, and the relative humidity is generally 95%.
Since animal cell culture fluids generally use a carbonate pH buffer system, and a certain concentration of CO2 is required in the culture environment, the culture of animal cells generally requires a CO2 incubator.
The working principle of carbon dioxide incubator:
First, the temperature control:
1. The temperature controller consists of Pt100 platinum resistance as the sensor and digital control circuit, LED digital display circuit, etc. When the sensor output is proportional to the temperature, the resistance signal is converted into a voltage signal and amplified by the circuit, and sent to the display circuit for display. temperature.
2. The other way is sent to the comparator and compared with the set value. When the two are biased, the thyristor power tube output power is triggered to generate heat in the heating tube. When the deviation is reduced to zero, the heat generated by the heating tube is also reduced until the heating is stopped.
3. There is over temperature and water level alarm function in the control line. When the display temperature exceeds the set value of 2.0 °C, the temperature controller emits an audible and visual alarm signal, and at the same time cuts off the output, stops heating, prevents the temperature from rising continuously; when the water level in the water tank is too high or too low, the water level controller emits an audible and visual alarm. Signal while cutting off the heating output. Only the water jacketed carbon dioxide incubator has a water level alarm function!
Second, the control of the gas path:
1, gas routing high concentration of carbon dioxide cylinders, air pumps, pressure regulators, needle valves, solenoid valves, flow meters and gas cylinders. (High-concentration carbon dioxide cylinders are provided by the user).
2. When a certain concentration of high-concentration carbon dioxide gas is input, the flow rate and time control of the regulating valve, the needle valve and the solenoid valve are controlled to ensure that a certain amount of carbon dioxide gas enters the working chamber, and the carbon dioxide concentration value in the working chamber is automatically controlled. This value is simultaneously displayed on the display circuit for easy observation. In order to facilitate the user to monitor and sample the concentration during use, a sampling and monitoring port is installed on the back side of the carbon dioxide incubator. Due to the adoption of the fast and slow double inflation method, after the carbon dioxide incubator is opened and closed, the concentration of carbon dioxide in the working chamber can be quickly recovered without overshoot.
3. During the working process of the carbon dioxide incubator, the working chamber is ventilated by the gas storage bottle to maintain a stable carbon dioxide concentration value. Third, the humidity control:
This machine is equipped with a water tray when the culture needs to maintain a certain humidity in the working chamber. The user can put the water tray into the working chamber during the cultivation, and the water tray is added with the appropriate amount of distilled water to naturally evaporate in the working chamber, and the relative humidity is generally 95%.
Since animal cell culture fluids generally use a carbonate pH buffer system, and a certain concentration of CO2 is required in the culture environment, the culture of animal cells generally requires a CO2 incubator.
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